Also, perform any required maintenance tasks as indicated by the instrument gauges and maintenance notifications on the data collection dashboard screen described in the applied biosystems 3500 3500xl genetic analyzer user guide part no. The 3500 series gas are developed with features useful for forensic scientists, including a normalization feature for analysis of the data designed to reduce the variation in peak height. Additionally, plate setup is simplified, as is built in primary analysis and quality control. Powerplexr 6c matrix standard technical manual tmd046. Applied biosystems 3500 genetic analyzer from thermo fisher. A validation study was performed for the 3500xl to carry out.
Data collection with the 3500 genetic analyzer is user friendly and intuitive, with simple dashboard navigation and easy to read displays. Rfid system of 3500 series which performs automatic detection of reagents and instrument accessories used, forces the instrument operators to replace these reagents and accessories unnecessarily frequently, nonefficiently and makes the whole operation of 3500 series very expensive. This validation was aimed to demonstrate that there is no effect on the applied biosystems 3500xl genetic analyzer thermo fisher scientific, uk after decommissioned and recommissioned at new premises. Abi maintenance, calibration, and supplies prepared by. The 3500xl 24capillary platform is also fluorescencebased utilizing capillary electrophoresis to support for sequencing application and fragment analysis. Audience this manual is written for principle investigators and laboratory staff who are. The results showed genotyping concordance which means using. Applied biosystems, user bulletin applied biosystems 35003500xl genetic analyzer, foster city, ca, 2011. Applied biosystems 3500 genetic analyzer from thermo. Biosystems 35003500xl genetic analyzer user guide part no. The 24capillary 3500xl genetic analyzer sets the standard in capillary electrophoresis.
Applied biosystems 35003500xl genetic analyzer user guide with 3500 series data collection software 3. Boot pc, login windows as 3500 user, wait for server monitor green check mark in the. They are all endoflife and endofsale starting in 2002. Lowlevel signal interference between samples separated on.
Same sample sets were tested on the abi 3500xl genetic analyzer at both old and new premises using current accredited procedures. Abi, summary of steps recommended order start instrument via power on button. Hid field applications specialist afdaa meeting austin, tx feb, 20. Compared with the previous 3 xl genetic analyzer 3 xl, the 3500xl has relative fluorescence units rfu that are significantly reformed because platforms such as the detection system and optical. The majority of applications can be run on a single polymer and capillary array.
The 3500xl system uses a 24capillary array and singleline 505 nm, solidstate longlife laser for significantly improved signal uniformity. Google scholar an internal validation of the applied biosystems 8capillary 3500 genetic analyzer was performed using two commercial short tandem repeat str multiplex kits with focus on setting appropriate thresholds for data analysis. Internal validation of the applied biosystems 3500xl genetic. Also, perform any required maintenance tasks as indicated by the instrument gauges and maintenance notifications on the data collection dashboard screen described in the applied biosystems 35003500xl genetic analyzer user guide part no. Guidelines for the validation process are published in the internal validation guide of. Troubleshooting common amplification and ce errorsissues april orbison sr. The purchase price of this applied biosystems 33 xl genetic analyzer includes a grant of a.
Mar, 2012 use of your genetic analyzer to determine the size of dna fragments making a multitude of fragmentbased applications possible on the same system as that for performing sanger sequencing. Applied biosystems 35003500xl genetic analyzer user guide 2010. Perform the spectral calibration as described in the abi 3500 3500xl genetic analyzer user manual. An overview of the powerplex fusion system protocol. Applied biosystems 3500 3500xl genetic analyzer, 3500 series. Dk ebook user manual guide referencenice ebook you should. Jan 23, 2012 working together, these elements provide the highest level of performance from a genetic analyzer to date. For additional information on performing spectral calibration, refer to the applied biosystems 35003500xl genetic. Applied biosystems 3500 and 3500xl genetic analyzers.
With this interface, users can clearly monitor the state of. Also, perform any required maintenance tasks as indicated by the instrument gauges and maintenance notifications on the data collection dashboard screen described in the applied biosystems 35003500xl genetic analyzer user. Applied biosystems 3500 genetic analyzer biosurplus. Applied biosystems microamp 96well support base 10 bases. The 3500xl dx genetic analyzer cs2 uses capillary electrophoresis and sanger technology to carry out accurate, reliable sequencing analyses.
For additional training and reference information, see the user documents provided with the instrument. Technical data sheet leukostrat cdx flt3 mutation assay. Oct 12, 2011 applied biosystems, user bulletin applied biosystems 35003500xl genetic analyzer, foster city, ca, 2011. Browse our useful resources and support for your 3500 or 3500xl genetic analyzer including setup and maintenance as well as troubleshooting advice. Two user attention words appear in applied b iosystems user. Compared with the previous 3 xl genetic analyzer 3. The 3500 series genetic analyzers set a new standard in capillary electrophoresis, with increased throughput, faster run times, and an innovative. Series genetic analyzer cathode buffer, anode buffer, and conditioning reagent are designed for readytouse, loadandrun installation. Applied biosystems 3500 series genetic analyzers for dna. Genetic analyzer 20 electrophoresis using the applied biosystems 33 xl genetic analyzer 28 electrophoresis using the applied biosystems 35003500xl genetic analyzer 38 analysis 50. Pdf lowlevel signal interference between samples separated. Offlabel uses such as fragment analysis are for research use only. View online or download applied biosystems 3500xl user manual. The complete product combines the instrument with applied b.
Your payment may be delayed if you do not follow the instructions noted below. Details as per annexure i 1,65,000 details as per annexure i total cip mumbai, price in usd one lac sixty five thousand only 1,65,000 important note. Feb 17, 2010 the 3500 series genetic analyzers set a new standard in capillary electrophoresis, with increased throughput, faster run times, and an innovative consumables system approach. Innogenomics technologies instruction manual igt 5dye matrix standard instruction manual version 3. Applied biosystems 3500 hid genetic analyzer 3500xl.
Rfid system of 3500 series which performs automatic detection of reagents and instrument accessories used, forces the instrument. The 3500 series data collection software integrates seamlessly with several downstream software packages to provide comprehensive analysis of genetic data. Applied biosystems 35003500xl genetic analyzer user bulletin. This instrument would be a fundamental component of the dna sequencing core facility, fulfilling the demanding research requirements that. Powerplexr fusion system for use on the applied biosystems. Applied biosystems 35003500xl genetic analyzer user guide applied biosystems, faster city ca. Dna fragment analysis by capillary electrophoresis user guide. Page 1 applied biosystems 35003500xl genetic analyzer user guide with 3500 series data collection software 3. Upgrade of applied biosystems genetic analyzers, 3500 series.
A new phenomenon was detected using the applied biosystems 3500xl genetic analyzer. Google scholar an internal validation of the applied biosystems 8capillary 3500. Bob morrison stlcccpls, instrumentation specialist initiated. The 3500xl genetic analyzer has increased the number of capillaries used, allowing more sample runs per injection while reducing the time of data output. Applied biosystems 35003500xl genetic analyzers support. Use of your genetic analyzer to determine the size of dna fragments making a multitude of fragmentbased applications possible on the same system as that for performing sanger sequencing. Genetic analyzers are automated systems capable of sequencing dna or analyzing fragments for a variety of applications. The most common ce systems used in forensic dna analysis include the abi prism 310, 3100, 3100 avant, 3, 3xl, 3500, and 3500xl genetic analyzers gas.
The 3500series instruments are the first genetic analyzers designed with a specific feature set and workflow for the. See user s manual or package insert for limited label license, and trademark information. Applied biosystems 35003500xl genetic analyzer with. It can collect data in ivd mode for diagnostic hla typing, as well as ruo mode. Details as per annexure i 1,65,000 details as per annexure i total cip mumbai, price in usd one lac sixty five thousand only 1,65,000 important. Powerplexr 5c matrix standard technical manual tmd049. Making second natureour first priorityit works the way that you work. The 3500xl offers multiple advantages over the 3xl genetic analyzers that are being used at the mspftc. User manual applied biosystems 3500xl user manual 326 pages. Anjali, colin, safety,mark sweeny, environmental, health. It can collect data in ivd mode for diagnostic hla typing, as. Applied biosystems microamp 96well support base 10 bases life sciences.
The system features an advanced longlife solidstate laser, which reduces the physical and environmental footprint of the system as well as the cost of ownership. The 3500 series genetic analyzers1 are specifically designed to support the demanding performance needs of validated and. Lowlevel signal interference between samples separated on the 3500xl capillary electrophoresis platform article pdf available in australian journal of forensic sciences 483. Manuals and user guides for applied biosystems 3500xl. Sequencing throughput and performance specifications. Applied biosystems 35003500xl genetic analyzer site.
Pcr products were separated and detected on an abi 3500 genetic analyzer using a 36 cm array and pop4 polymer applied biosystems. With this interface, users can clearly monitor the state of consumables and schedule maintenance protocols. Hence this instrument would be a jointuse instrument, used in a variety of projects across several investigative. We set a new standard in capillary electrophoresis with the 8capillary 3500 and 24capillary 3500xl genetic analyzers. Applied biosystems 3730 or 3730xl dna analyzers 3730 series. Page 1 applied biosystems 3500 3500xl genetic analyzer user guide with 3500 series data collection software 3. Nist validation studies on the 3500 genetic analyzer. Troubleshooting common amplification and ce errorsissues. Estimating allele dropout probabilities by logistic. Anjali, colin, safety,mark sweeny, environmental, health and. The 3500xl genetic analyzer will be used for short tandem repeat str analysis because 3xl genetic analyzer has been discontinued. Applied biosystems 35003500xl genetic analyzer user guide pn.
A genemapper idx software abi prism 3100 or 3100avant genetic analyzer section 5. Applied biosystems 3500xl manuals manuals and user guides for applied biosystems 3500xl. Advanced multiplex capabilities for dna fragment analysis containing up to six unique dyes. Applied biosystems 35003500xl genetic analyzers support thermo fisher scientific us. Identifiler and identifiler plus thermal cycling protocols followed manufacturer recommendations found in each kits manual, and was carried out on the geneamp 9700 thermal cycler applied biosystems. Upon installation of a new 3500 series instrument for hid analysis, and prior to. Use of the msi analysis system with the applied biosystems. We have 1 applied biosystems 3500xl manual available for free pdf download. Use of the msi analysis system with the applied biosystemsr. B genemapper id software applied biosystems 3 or 3xl genetic analyzer section 5. Conditioning reagent for 3500 dx3500xl dx genetic analyzers. Applied biosystems 35003500xl genetic analyzer user.
In capillary electrophoresisbased systems, dna fragments bound to probes migrate through a polymer and the fluorescence emissions are measured. This phenomenon is characterised by a strong sample in an injection imparting lowlevel peaks to negative. Dna sequencers genetic analyzers genetic analyzers are automated systems capable of sequencing dna or analyzing fragments for a variety of applications. Details of purchase order of applied biosystems 3500xl. Use of the geneprintr 10 system with the applied biosystems. The 3500series instruments are the first genetic analyzers designed with a specific feature set and workflow for the human identification application. Powerplex esi 17 pro system technical manual, tmd036. Specification sheet 3500 and 3500xl series systems overview applied biosystems sets a new standard in capillary electrophoresis with the 8capillary 3500 and 24capillary 3500xl genetic analyzers. Applied biosystems 3500 or 3500xl genetic analyzer section 5. Multichannel pipettes, manual and electronic centrifuge capable of x g with a swingout rotor and refrigeration centrifuge capable of 1400 x g with a swingout rotor benchtop mini microcentrifuge.
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